IGEM:British Columbia/2009/Notebook/Biosensor Sensitivity/2009/07/29
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cPCR of Lock/Key/Control-lock into Ampicillin and Chloramphenicol construction plasmids (from July 28) to verify constructs
Forward (FW): VF2 Reverse (RE): VR2
plates from July 28
Plates with heat inactivation were sealed (parafilm) and stored at 4°C. Same with the transformants without heat inactivation of digest.
Making Minimal Media
NOTE: Two batches of M9 Salts were made. The first batch was made using anhydrous Na2HPO4. In order to keep the molarity of the final solution the same, 33g of anhydrous Na2HPO4 was used instead of 64g Na2HPO4.7H2O. The second batch of M9 salts were made with correctly hydrated salts.
NOTE: 3 batches of M9 Minimal Media was made. The first (M9 Minimal Media ‘A’), had non filter-sterilized arabinose added to it and was autoclaved at the end to sterilize it. After autoclaving, the arabinose caramilized and the solution turned a light brown. There was also some precipitation in the bottom of the flask. The second (M9 Minimal Media ‘B’) was made with the first batch of M9 salts containing anhydrous Na2HPO4. The third (M9 Minimal Media ‘C’) was made with the other batch of M9 Salts containing correctly hydrated salts.
Overnights with M9 Minimal Media