Results of M9 Minimal Media Test
- The results of growing pBADweak+GFP, pBADwt+GFP, J23100 and J23101 in 2 different batches of arabinose-glycerol M9 Minimal media
Results of M9 Minimal Media B
- The cultures of pBADweak+GFP, pBADwt+GFP and J23100 showed turbitity, but a noticable color change was not apparent
- The culture of J23101 had a light color change, but did not show much turbitity
Results of M9 Minimal Media C
- All cultures showed growth
- The cultures containing J23100 and J23101 were bright red. J23100 was a little bit darker then J23101, which is consistant with the Registry as J23100 is a stronger promoter
- pBADstrong+RFP was slightly red, consistant with the results of the constituent promoters as pBAD is a weaker promoter
- pBADwt+GFP and pBADweak+GFP had cell growth but were not noticibly green, consistant results as the GFP is LVA tagged and will degrade faster then RFP
Conclusions
- The M9 Minimal Media C had more consistant results then B, therefore will only use C batch fro future experiments.
|