IGEM:British Columbia/2009/Notebook/Biosensor Sensitivity/2009/08/10

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Innoculation

innoculated overnight cultures from plates (5mL LB + appropriate antibiotic) + spot plate -> ASEM10, 11, [LOX]R, Cre-LVA

  • note: innoculation in triplicate with appropriate negative control

sequencing results of ASEM 17, 18, 19, 20, 21

all except ASEM 18: good alignment with expected sequence (91.03 - 99.27%)

Diluted DNA stocks from Amelia's batch


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