IGEM:Cambridge/2008/Notebook/Bacillus/2008/09/05

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Starch plates

Prepare some starch plates. We want to test our transformation but we can not manage to PCR Bacillus. So we are going to try to extract chromosomal DNA from Bacillus. We make these starch plates to test it with colonies which have positive control with amylase test.

- Prepare starch plates (you can find the protocol here)

- Plate 4 different plates :

  • 2 colonies of IA75 (positive control) and 3 colonies of IA771 (negative control)
  • 5 colonies of IA751 transformed with ECE153 from 29/08
  • 3 colonies of IA751 transformed with ECE153 from 02/09 and 3 colonies from 03/09
  • 5 colonies of IA751 transformed with ECE112 from 02/09



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