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Brief Introduction of Fur
Iron is an important nutrient for E.coli but it presents problems of toxicity at high concentration, poor solubility and low availability. Escherichia coli achieves iron homeostasis through regulation of numerous iron acquisition systems and iron storage proteins. This regulation is mediated by the Fur (ferric uptake regulation) protein, which generally acts as a repressor that utilises ferrous iron as a co-repressor. Fur also regulates genes involved in other functions such as acid shock response, defence against oxygen radicals, and virulence, and is therefore regarded as a global regulator. Thus, fur- mutants which have higher free iron ion concentration in the cytoplasm are to be used for the formation of magnetite in vivo.
Preparing fur- Mutants Colonies
Gene (mms6 or mamC):
Plasmid pSB3K3: http://partsregistry.org/Part:pSB3K3
Restriction Digestion of I0500 and pSB3K3