IGEM:Cambridge/2008/Notebook/Turing Pattern Formation/2008/08/06
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PCR parts of Agr operon
We received primers for Agr A,B,C, and D today - http://openwetware.org/wiki/IGEM:Cambridge/2008/Turing_Pattern_Formation/Primers
We must PCR each individual part out of the previous BioBricks (I746001 &I746101) in order to put our bacillus RBS on them.
I was concerned that the region of most homology would be the BioBrick prefix and suffix itself, so I first digested the DNA with Xbal and SpeI.
For some reason the digestion didn't work that well, so I've decided to go ahead and use the uncut BioBrick DNA as template for my reactions.