IGEM:Cambridge/2008/Notebook/Turing Pattern Formation/2008/09/11
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- We run our PCR products from yesterday on a new 1.8% agarose gel
- Nothing, except for the PCR with VF and VR primers, either our primers are not right, either we have no insert.
RBS screning but single digest
- Grow some colonies of Top10 transformed with RBS into 10mL of LB with antibiotic
We want to check our transformation with single digest. If we have self ligation in our transformation, we will loose the XbaI cutting site.
Backbone for ligation
- New pellets in 600μL of SDW
- Plasmid miniprep