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  • The liagtion reaction from last week was drop dialised and then 5μL used to transform highly competent TOP10, before plating on chloramphenicol plates.
  • The Biobrick combination of promoter-RBS were then cut with Pst1 and EcoR1 and ligated into pSB4C5 and then transformed into TOP10 and incubated overnight at room temperature in 600μL SOC.

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