IGEM:Cambridge/2008/Notebook/Voltage/2008/08/27

osmY RBS

• Primers containing RBS for osmY Biobrick were received
• PCR was performed from biobrick J45250
• Ligated into dephosphorylated vector backbone
• Colonies transformed

Restriction Digest & Ligation Test

• Various combinations of restriction digests & T4 phage genome ligation test.
• All results positive :-)