IGEM:Groningen/2009/Notebook/iGEM 2011/2011/07/06

iGEM Project name 1

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Entry title Colony PCR of 6 constructs! HybB promotor in pSB1CR HybB promotor in RBS GFP ampicillin resistance vector PcI promotor in RBS GFP ampicillin resistance vector PlasI promotor in RBS GFP ampicillin resistance vector LasI-LVA in ampicillin resistance with double terminator vector cI-LVA in ampicillin resistance with double terminator vector Colony PCR protocol: Media: Taq PCR mastermix Preheated Lid: 111° C Denaturation: 95 ° C for ten minutes Cycle 33× Denaturation: 95° C for 30 seconds Annealing: 60° C for 30 seconds Extension: 72° C for 1 minute Final extension: 72° C for ten minutes Store at 4 ° C (infinite)