IGEM:Harvard/2006/Cyanobacteria/Notebook/2006-10-14

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CIP treatment (fail)

CIP treated the '0S' sequence, which is J36010/SP. Will need to re-CIP treat.

Gel extraction

Image: firstgel_101406.jpg

  • 1: Ladder
  • 2: undigested 36010
  • 3: digested 36010 /XP marker
  • 4: digested 36010 /XP
  • 5: digested 36010 /XP
  • 6: digested 36010 /XP (not shown)
  • 7: digested 36010 /XP (not shown)
  • 8: digested 36010 /XP marker
  • 9: Ladder
  • 10: digested 36011 /SP
  • 11: digested 36011 /SP (not shown)
  • 12: Ladder
  • 13: digested 36010 /SP marker
  • 14: digested 36010 /SP
  • 15: digested 36010 /SP
  • 16: digested 36010 /SP
  • 17: digested 36010 /SP
  • 18: digested 36010 /SP marker
  • 19: undigested 36010
  • 20: Ladder

The gel ran as if there was a lot of genomic DNA; additionally there is no KaiC /XP expected 1.8kb insert in lanes 3-5; and notice in lane 10 that the supposed KaiB /SP is a smudge.

Will poststain next gel to verify results.

Gel#2:


Image: 36010_zs_101406.jpg

  • 1: ladder
  • 2: undigested 36010
  • 3: undigested 36011
  • 4: digested 36011 /sp marker
  • 5: digested 36011 /sp (not shown)
  • 6: digested 36011 /sp (not shown)
  • 7 digested 36011 /sp marker
  • 8: ladder

Innoculation

Reinnoculated M27 and B18.

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