IGEM:Harvard/2006/Cyanobacteria/Notebook/2006-10-16

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Running digest products on a gel

Hetmann yesterday digested 10 \xp, 11\sp, and 11\xp. Running them on a gel after CIP treatement of the 11\sp...

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Note: Expected values

  J36011\XP: 550, 3200
  J36011\SP: 3700
  J36010\XP: 1800, 3200
  J36010\SP: 5000

Running diagnostic egel

Image: egel_Zs101606.jpg 1: 1kb+
2: 10 undigested from lastest failed midiprep
3: 10 undigested from the midiprep we KNOW worked
4: 10 undigested from previous failed miniprep
5: 11 undigested from latest failed midiprep
6: 11 undigested from previous failed midiprep
7: 11 undigested from the sequencing miniprep, KNOW worked
8: 11 \xp from today
9: 11 \sp from today
10: 1kb+

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