IGEM:Harvard/2006/Cyanobacteria/Notebook/2006-8-17

Gel image of Kai\X-P, J04500\S-P, J04500\X-P, and B0034\S-P

Ligation and Transformation attempt #3 for stage 1 of constructs

This ligation and transformation attempt was done for 9 things:

• B0034\sp + KaiA \xp, KaiB \xp, KaiC \xp
• J4500\xp from the 97.3 ng/uL midiprep + KaiA \xp, KaiB \xp, KaiC \xp
• J4500\xp from the 97.3 ng/uL midiprep + KaiA \xp, KaiB \xp, KaiC \xp

Did a 1:3 volume ratio because the gel cutout did not give good nanodrop numbers; the usual, 25uL competent cells per sample, plated 5 on KAN (the J4500 \xp), 5 on Carb (the B0034 and the controls), and 1 of the J4500\xp on KAN (the KaiC \xp).