Cyano continues! And you all thought we were dead...
- Chiba letter
- Miniprep Nick's plates and sequence
- Begin taking aliquots of M2-7 J04500+KaiC at time points
- The other (less than 6) stage1 ligations
Verification of J04500+KaiC
Based on the (20!) sequencing results from way back in August (how is it freezing now...)I:
- Created a file on MCB1001 (small room, left computer), ZS_J04500+kaiC which contains the expected plasmid we want
- Aligned all 10 VF2's to the expected file
- Tested fully one of them, M2-7
And we have the correct plasmid! First biobrick...