IGEM:Harvard/2006/Stock solutions

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Contents

Salts

Calcium chloride

To make 100 mL of 0.10 M:

  • 1.47 g CaCl2 · 2H2O
  • add dH2O to 100 mL

Magnesium chloride

To make 100 mL of 0.10 M:

  • 2.03 g MgCl2 · 6H2O
  • add dH2O to 100 mL

Potassium chloride

To make 100 mL of 1.0 M:

  • 7.46 g KCl
  • add dH2O to 100 mL

Sodium chloride

To make 100 mL of 1.0 M:

  • 5.84 g NaCl
  • add dH2O to 100 mL

Standard buffers

Tris-acetate

To make 100 mL of 2.0 M:

  • 24.2 g Tris
  • add dH2O to 90 mL
  • add glacial acetic acid until desired pH (pH 7.4 is about 7 mL of acid)
  • add dH2O to 100 mL

Mixed buffers

Bock's selection buffer

To make 40 mL of 5x:

  • 2 mL of 2 M Tris-acetate pH 7.4 (final concentration: 100 mM)
  • 28 mL of 1 M NaCl (final concentration: 0.7 M)
  • 1 mL of 1 M KCl (final concentration: 25 mM)
  • 2 mL of 0.1 M CaCl2 (final concentration: 5 mM)
  • 2 mL of 0.1 M MgCl2 (final concentration: 5 mM)
  • 5 mL dH2O
Reference:
  1. Bock LC, Griffin LC, Latham JA, Vermaas EH, and Toole JJ. . pmid:1741036. PubMed HubMed [bock]

Bittker binding buffer

(buffer used by Bittker et al. in selecting for aptamer that binds streptavidin)

  • 150 mM NaCl
  • 50 mM Tris HCl pH 8.0
  • 10 mM MgCl2
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