IGEM:Harvard/2006/Supplmented agarose gel

From OpenWetWare

Jump to: navigation, search

2% agarose gel:

  • 99 mL TBE
  • 1 mL 1 M MgCl2 (to supplement to 10 mM)
  • 3 μL 10 mg/mL EtBr (to supplement to 300 μM)

run in:

  • 594 mL TBE
  • 6 mL 1 M MgCl2 (to supplement to 10 mM)

loading dye:

  • 1 μL 10x TBE/glycerol w/ bromphenol blue added to each well
Personal tools