IGEM:Harvard/2006/vlau/Notebook/2006-7-13

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Protein & DNA-staining Experiment (Repeat 1)

1. Rxn Mixtures

Tube Lane Thrombin mass (μg) 2 μM thrombin (μL)
(2 μM = 1.299 μg/μL)
water (μL) 10x loading dye (μL) total volume (μL)
180.650.56.53.010.0
291.301.06.03.010.0
3101.951.55.53.010.0
4112.692.05.03.010.0
Tube Lane 2 μM Aptamer (μL) water (μL) 10x loading dye (μL) total volume (μL)
121.06.03.010.0
232.05.03.010.0
343.04.03.010.0
454.03.03.010.0

2. Protocol

   - gel run @ 120V for 1.25 hrs. 
   - gel cut into DNA & protein sections after removal from cartridge (slight tearing towards bottom)
   - protein section stained w/ GelCode Blue Stain (Coomassie Blue)  for 1 hr.
   - DNA section stained w/ 10μL EtBr in 100 mL Tris-glycine buffer for 1 hr.
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