IGEM:Harvard/2008/Lab Notebooks/DailyBook/Week14/Chemical and Light

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Mystery Colony PCR 10/08

Colony PCR of putative fungal colonies using primers BBpfx and mtrB500.

Image:10-08_Mystery_PCR_MXHTA.jpg

Lane 1: 1 kb ladder

Lanes 2-6: P136 plate (500 bp)

Lane 7: P140 plate (1.9 kb)

Lanes 8-12: P122>131 plate (1.3 kb)

Colony PCR 10/11

Unadulterated gel

Image:10-11_Colony_PCR_MXHTA.jpg


Image:10-11_Colony_PCR_1-12.jpg‎

Lanes 1-2: P148

Lane 3-4: P147 (picked 3)

Lanes 5-12: 147 (picked 6)

Image:10-11_Colony_PCR_13-24.jpg‎

Lanes 13-14: P147

Lanes 15-24: P146 (picked 17, 23)

Image:10-11_Colony_PCR_25-36.jpg‎

Lanes 25-26: P146

Lanes 27-36: P144 (picked 30, 35)

Image:10-11_Colony_PCR_37-48.jpg‎

Lanes 37-39: P144

Lanes 40-48: P145 (picked 41, 46)

Ligations 10/10

' Part 1 Part 2 Part 3 Notes ' Total Volume Growth
Ligation 1123m4 ES131 XPP1 EPts in Kanjust chem tran
84315
Ligation 2122m4 Es131 XPP1 EPts in kanjust chem tran
84315
Ligation 338+130 #20 ESX mmIP1 ES
2ul to tube3
Ligation 439+130 #31 ESX P1 ES
10ul to insert tube35
Ligation 5142 EsP1 ES
10ul to insert tube23
Ligation 6138 #2 ESP1 ES
10ul to insert tube20
Ligation 7137 #32 ESP1 ES
10ul to insert tube31
Ligation 8140 ESP1 ES
10ul to insert tube20
Ligation 9131 XP116 SP
448
Ligation 10131 XP117 SP
448
Ligation 11131 XP108 Sp
448
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