IGEM:Harvard/2009/Notebook/Harvard iGEM 2010/2010/06/14
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BioBrick parts from the 2010 iGEM kit were transformed and grown in highly competent TURBO bacteria.
BBa_J45700 - entire pathway, Ampicillin
BBa_J45250 - ATF3 + Promoter, Ampicillin?
Performed bacterial transformation according to Silver:_Bacterial_Transformation, however we used 1μL in step 3, and used entire tube of TOP10 competent cells instead of only 10-15μL.
Transformed the following, each into its own tube of TOP10 E.Coli:
1) LacI with a rapid degredation tail attached, biobrick BBa_C0012, located on plate 1, well 2O, plasmid pSB1A2
2) LacI wildtype, biobrick BBa_I732100, located on plate 2, well 10E, plasmid pSB1A3
Streaked 100μL each of 1 and 2 onto separate LB + Amp dishes, along with a control dish of 100μL untransformed TOP10 E.Coli cells.
Plates incubated overnight, colonies observed in all plates except the control.