IGEM:Harvard/2009/Notebook/Harvard iGEM 2010/2010/08/09

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Team Allergy

  1. Redigest V9/V10 (three digests of each backbone to use more DNA in ligations)
  2. Grew up V9/V10+ ihpRNA colonies
  3. Re pcrd amiRNA and digested to ligate into V9/V10
  4. Catalog parts to be ready for the registry

Team Fence

Gel Extracted Friday's Digests

Nanodrop of Friday's Digest Gel Extractions

Plasmid Quantity (ng/μL) 260/280
Barstar Eco Pst 1 .9 -.57
Barstar Eco Pst 2 -.4 .14
RXRLc Xba Pst .3 -.19
Barnase Eco Pst 1 20.5 1.64
Barnase Eco Pst 2 14.4 1.83
5xGalUAS Spe Pst 1 10.7 1.87
5xGalUAS Spe Pst 2 16.4 1.90
pENTcup Spe Pst 1 16.3 1.88
pENTcup Spe Pst 2 14.7 2.09
PhyB Bam Nco 1 1.7 -1.10
PhyB Bam Nco 2 -.6 .24
V26 Eco Pst 1 2.4 1.6
V26 Eco Pst 2 1.3 1.05
V26 Eco Pst 3 1.4 3.01
V24 Eco Pst 1 8.3 3.6
V24 Eco Pst 2 5.5 1.64
VP16 Eco Pst 1 3.4 1.6
VP16 Spe Pst 1 9.4 1.92
VP16 Spe Pst 2 36.9 1.73
RXRLc Bam Xho 1 7.7 2.51
RXRLc Bam Xho 2 4.1 1.93
35s min anneal 1/100 from 8/2 3.6 1.31
RXRLc Xba Pst 3.6 1.31


Redo of some digests with no insert where there should have been an insert Image: 89moredigestspm.jpg

  • ladder
  • pACT2 (backbone)
  • EcRGal (insert)- STILL NO INSERT
  • RxRLC (insert) Xba and Pst
  • RxRLC (insert) Bam and Xho



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