IGEM:Harvard/2009/Notebook/Harvard iGEM 2010/2010/08/12

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Team Allergy ihpRNA Minipreps of more V9/V10 colonies Diagnostic Digest of minipreps w/ Eco and Pst (Should see ~840 and 7000 bp) ladder, V92, V92b, V94, V94b, V95, V96b, V97, V97b, V98, V98b Lanes 2&3: LTP (11c1); Lanes 4&5: Bet2 (25c1); Lane 6: Bet2 (25c2); Lane 7: Bet2 (28c1); Lanes 8&9: Ger (36c1); Lanes 10&11 Ger (36c2) Digested V9/V10 backbone for ligations w/ Eco & Pst (redoing ihpRNA ligations into V9/V10) amiRNA Ligated into V9/V10 and transformed (mutagenesis sites confirmed through pcr using 20 bp sepecific primers) still need to be sequenced [[Image: Team Fence This picture is not the best picture but we did find colonies on the plate with the arp2/b21 transformation. transformation from karmella's stock Transformations from Yesterday's Ligations Colonies beyond control on RXRLc VP16, Barstar V26, and EcR-Gal-PhyB.