IGEM:Harvard/2010/Brainstorming/food

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Contents

Strains, Plasmids, and Trasformation

references

1. Broothearts et. al. Gene transfer to plants by diverse species of bacteria. Nature, 2004.

2. Galvin [1] Agrobacterium-Mediated Plant Transformation: the Biology behind the “Gene-Jockeying” Tool

Agrobacterium infection diagram

3. Antunes, et. al. [2] A synthetic de-greening gene circuit provides a reporting system that is remotely detectable and has a re-set capacity

4. Everything you want to know about Arabidopsis [3]

protocols

RNAi

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Markers and Genetic Fences

Dual selection system ideas

  • use selectable markers such as URA3 to control whether an integration plasmid is replicated or not
  • cells with the URA3 gene can grow on media without uracil, but get killed by 5-Fluoroorotic acid (5-FOA)
  • cells without the URA3 gene die on media without uracil, but can survive exposure to 5-FOA


De-Greening



Downstream effectors

allergens

Peanut and Tomatoes review

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Peanut profilins: Ara h knock-down

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Tomato profilins: Lyc e knock-down

  1. Design of tomato fruits with reduced allergenicity by dsRNAi-mediated inhibition of ns-LTP (Lyc e 3) expression. Le LQ, Lorenz Y, Scheurer S, Fötisch K, Enrique E, Bartra J, Biemelt S, Vieths S, Sonnewald U. Plant Biotechnol J. 2006 Mar;4(2):231-42. pmid:17177799
  2. Reduced allergenicity of tomato fruits harvested from Lyc e 1-silenced transgenic tomato plants. Le LQ, Mahler V, Lorenz Y, Scheurer S, Biemelt S, Vieths S, Sonnewald U. J Allergy Clin Immunol. 2006 Nov;118(5):1176-83. Epub 2006 Sep 8. pmid:17088146

Orange profilin: Cit s 2 (characterized, but not knocked down yet)

  1. Isolation, cloning and allergenic reactivity of natural profilin Cit s 2, a major orange allergen. López-Torrejón G, Ibáñez MD, Ahrazem O, Sánchez-Monge R, Sastre J, Lombardero M, Barber D, Salcedo G. Allergy. 2005 Nov;60(11):1424-9. pmid:16197476

flavors

  • [4] Tieman et al. Tomato aromatic amino acid decarboxylases participate in synthesis of the flavor volatiles 2-phenylethanol and 2-phenylacetaldehyde

VANILLA: http://www.3dchem.com/moremolecules.asp?ID=307&othername=Vanilla#

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De novo biosynthesis of vanillin in fission yeast (Schizosaccharomyces pombe) and baker's yeast (Saccharomyces cerevisiae). http://www.ncbi.nlm.nih.gov.ezp-prod1.hul.harvard.edu/pubmed/19286778

miraculin

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spices

Capsaicin: http://en.wikipedia.org/wiki/Capsaicin

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From wikipedia:

others

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http://en.wikipedia.org/wiki/Limonene

caffeine etc.

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  1. http://www.plantcell.org/cgi/content/abstract/6/5/723 [2]


Questions

can we eat it for the iGEM project (human testing)? Can we hand it out to be eaten (FDA)? Patent/copyright issues? Can we have actual tranformed plants ready by the jamboree

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