IGEM:IMPERIAL/2006/LabCalendar/10aug06

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Lab Update 10th August

This Report is a summary for full details see the lab notebook

Aims For Friday

  • Finish Stage three Ligations (With the exception of the aiiA part)
  • Finish Stage two ligations (by wedensday)
  • Test part T9002 (This part is the AHL/GFP assay and the Predator AHL sensor)

Aims For today

  • Maxiprep and electroporate for ligations John SY
  • Retry PCR Cre Lox part John C
  • PCR Immunotag Deepti and johhny

Current Ligation Status

  • Tick = Done and tested
  • ? = Ligated and untested
  • X = Done and Failed we will retry

Image:Ligation chart 10 aug.JPG

Issues

Riboswitch

  • Unable to tell if worked from PCR
  • Will be sequenced (takes 2-3 days) waiting on result

PCR

  • The PCR results do not correlate with the miniprep results
  • PCR for immunotag and lox part is not working

minipreps

  • These are not conclusive for many parts, we are looking into using a restriction digest

John Chattaway (Lab Co-ordinator)

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