IGEM:IMPERIAL/2006/LabCalendar/2006-7-18

• IGEM:IMPERIAL/2006/LabCalendar/2006-7-18 Events
• Day in the lab
• Project Progress

Identification and extraction of parts

We have already successfully extracted and cultured 4 parts (All from Plate 1)

• I13272 well 4F
• F2621 well 4N
• I13273 well 4H
• I13208 well 4J

A further 16 parts were identified as being necessary for use in our project. (From Plate 1 unless stated)

• F2620 well 6B
• I0460 well 11I
• F1610 well 1B (Plate 2)
• E0040 well 5H
• T9002 well 19B (Plate 2)
• I13207 well 2P
• I0403 well 5P
• C0261 well 24A
• E0434 well 20J
• R0062well 9G
• B0034well 3O
• C0062well 7A
• I13504well 12D
• I14032 well 17K (Plate 2) (Kanomycin resistant plasmid, cultured in Kanomycin plate)
• C0260 well 24C
• C0160 well 9F

Electroporation

Preformed electroporation on competent cells with the following plasmids and set in 37C incubator overnight to grow. All except 17K were incubated on ampicillin plates since they all had ampicillin resistance gene.