IGEM:IMPERIAL/2006/LabCalendar/2006-7-30

• IGEM:IMPERIAL/2006/LabCalendar/2006-7-30 Events
• Day in the lab
• Project Progress

Cultures

• 2 Cultures of 1I
• 6 cultures of 12D->2H
• 6 cultures of 12D->24A
• 2 cultures of 7A->3O (only 2 cultures on plate)

All cultures in 2 mL LB Amp

To do for Monday 31/7

• Find out expected digest lengths
  • Part 1I (B0015) part length = 129 bp, plasmid length = 3189 bp (pSB1AK3)
  • Part 12D->2H (I13504 insert -> I13033 vector) part length = 875 + 192 = 1067 bp, plasmid length = 3189 bp (pSB1AK3)
  • Part 12D->24A (I13504 insert -> C0261 vector) part length = 875 + 661 = 1536 bp, plasmid length = 2079 bp (pSB1A2)
  • Part 7A->3O (C0062 insert -> B0034 vector) part length = 756 + 12 = 768 bp, plasmid length = 2079 bp (pSB1A2
• Miniprep above cultures