IGEM:IMPERIAL/2006/LabCalendar/2006-7-30

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Cultures

  • 2 Cultures of 1I
  • 6 cultures of 12D->2H
  • 6 cultures of 12D->24A
  • 2 cultures of 7A->3O (only 2 cultures on plate)

All cultures in 2 mL LB Amp

To do for Monday 31/7

  • Find out expected digest lengths
    • Part 1I (B0015) part length = 129 bp, plasmid length = 3189 bp (pSB1AK3)
    • Part 12D->2H (I13504 insert -> I13033 vector) part length = 875 + 192 = 1067 bp, plasmid length = 3189 bp (pSB1AK3)
    • Part 12D->24A (I13504 insert -> C0261 vector) part length = 875 + 661 = 1536 bp, plasmid length = 2079 bp (pSB1A2)
    • Part 7A->3O (C0062 insert -> B0034 vector) part length = 756 + 12 = 768 bp, plasmid length = 2079 bp (pSB1A2
  • Miniprep above cultures
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