IGEM:IMPERIAL/2006/LabCalendar/2006-8-13

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Contents

To do today

  • Miniprep (37015RS & J37016)
  • Reculture and miniprep cre 1, 2, 4 & AiiA
  • Culture T9002 on request by Tom (2 mL M9, 2 mL LB)

Re-miniprep (J37015RS & J37016)

  • L2, 3, 4
  • M1, 2
  • V1, 2, 3
  • R1, 2
  • Miniprep product in fridge to be digested tomorrow (pending acquisition of PstI and more EcoR1)
  • 1 Day delay expected

Cultures

  • Culture in Liquid Media
    • Cre 1, 2, 4 (2 colonies each)
    • AiiA (4 colonies)
    • 12D->2H (4 colonies)

End of Sunday

  • Cultured Cre 1, 2, 4, AiiA and 12->2H in 100ml for maxiprep tomorrow
  • Cultured T9002 in 2ml of LB and M9 for testing tomorrow
  • Failed to miniprep Cre 1, 2, 4, AiiA and 12->2H

To do tomorrow

  • Complete digestion of J37015RS, J37016, Cre 1, 2, 4, AiiA, 12D->2H and run gel (from miniprep)
  • Maxiprep Cre 1, 2, 4, AiiA, 12D->2H in parallel with
  • T9002 testing
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