IGEM:IMPERIAL/2006/LabCalendar/2006-8-2
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M9 Broth has been made up and is sitting in the fridge in T/L
Electroporations
- We electroporated the following in the morning
- I13521, 18I
- 7A->3O DNA 2
- DNA and competent cells with DNA are in cold room
- Plates in 37C incubator in T/L
Maxiprep
- Maxi-preped the following parts
- 1I
- 5M
- 12D->24A colony 1, 3, 4
- 16P
- 2M
- 7A->3O colony 2
- Maxiprep DNA in fridge in T/L
Ligation
- Redo ligation 12D->2H
- Cut 12D with P & X, yellow buffer
- Cut 2H with S & P, orange buffer
- 30 uL digests
- extract DNA successful, however, there was a faint larger band in the 2H lane, but we took out the larger band which corresponded most closely to 3381 bp, the expected size
- Gene clean wash
- Set ligations at 14:30, to be ready at 16:30
- Gene clean ligation product
- Electroporation completed
- Incubated at 37C in Dr. Mann's Lab
Electrocompetent cells
- Cells have been made competent...see protocol