IGEM:IMPERIAL/2006/LabCalendar/2006-9-9

From OpenWetWare

Jump to: navigation, search



TO DO:
*Maxi & mini of new J37024 (1,2,3,4) and J37019
*Ligations & Electroporations: J37025 & J37022
*Testing:J37015
*Culture: J37015, J37019 and J37016 for J37015 testing

Testing J37015

Issues

  • Fresh cultures of 015, 019 & 016 failed to be made yesterday so testing is being conducted from the 10ml culture of 015 & 019 from the 8/9/06 and the remainder of the 2ml culture from 7/9/06.


  • OD600 of J37015: 2.0 reculture 0.5ml into 10ml
  • OD600 of J37019: 1.864 reculture 0.536ml into 10ml
  • OD600 of J37016: 2.0 reculture 0.8ml into 16ml
  • Placed in the shaker at 10:40, take out at 12:40.

Ligations

  • Maxied new J37024 (1,2,3,4) and J37019
  • Ligated J37025 & J37022 and electroporated
Personal tools