IGEM:IMPERIAL/2006/LabCalendar/8aug06

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Contents

Lab Update 8th August

This Report is a summary for full details see the lab notebook

Aims For Friday

  • Finish Stage three Ligations (With the exception of the aiiA part)
  • Finish Stage two ligations (by wedensday)
  • Test part T9002 (This part is the AHL/GFP assay and the Predator AHL sensor)

Aims For today

  • Order Supplies John SY
  • Maxiprep and electroporate for ligations Deepti, Farah, Johhny and John SY
  • Test part T9002 Tom
  • PCR Cre Lox part John C

Current Ligation Status

  • Tick = Done and tested
  • ? = Ligated and untested
  • X = Done and Failed we will retry

Image:Pic.JPG Image:Ligation Chart8aug 2006.ppt

Issues

Riboswitch

  • Unable to tell if worked from PCR
  • Will be sequenced (takes 2-3 days)

PCR

  • The PCR results do not correlate with the miniprep results

minipreps

  • These are not conclusive for many parts, we are looking into using a restriction digest

John Chattaway (Lab Co-ordinator)

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