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Open Issues

  • Need to double check ligation chart !!!
ligation chart for test constructs
ligation chart for test constructs


  • The parameters of the oscillator are based on the OD of our 2 cells, their ratio and the volume of the culture.
    • do we want to keep constant the growth ? How ?
    • At which OD do we want the oscillator to operate ? which growth phase (exp, stationary ...) ?
    • Is the use of a chemostat compatible with Lotka-Volterra dynamic ?
    • In which media do we want to use the oscillator (LB, M9) and do the characterization ?
  • Can we have access to Wallac Victor 3 equipement for characterization ? (ask Prof Freemont)
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