IGEM:IMPERIAL/2006/Protocols/western blot
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Western Blot Protocol
- Grow overnight in a 5 ml culture
- Make a fresh day culture 400μL of overnight culture + 4.6 ml of fresh medium (LB)
- Grow fresh culture for 1.5hrs
- Remove 1ml (This will be your control)
- Add IPTG to the remaining 4ml - Final conc should be 0.5-1 milimolar (So add 4μL of 1 molar IPTG)
- Grow for 2-3 hrs
- Centrifuge down
- Add Lysis Buffers
- Run on gel
You should get this