# IGEM:IMPERIAL/2006/project/Oscillator/Theoretical Analyses/Results/2D Model4

Model 4:Bounded Predator and Prey Growth with Regulated killing of Preys

# Introduction

Generalities

• After proving that bounding both growth and enzymatic degradation of the preys grants the system the capacity to oscillate
- we now reintroduce the bounded predator growth
- and check that is does not disturb the balance to the extent that oscillations are impossible again.
• The Dynamical System of Interest in this section is:

Physical interpretation of the equations

• From a physical point of view the equations are those of the molecular predation system in the absence of chemostat (or equivalently with zero washout).

Dimensionless Model

• Since we now have 7 variables , we normalise the system by rescaling the X,Y axes and changing the time scale as we did with model 3, which leaves us with 4 variables only.
• We will use this dimension-less version of the Dynamic System for the rest of the study.

# Basic Results on the Steady Points

• The origin (0,0) remains a steady point
• The other steady points of the system are associated to a quadratic equation

• The origin (0,0) remains a saddle point
- One of them is a saddle point
- The nature of the other point can be adjusted (from stable to unstable via centre)
• If BC/D is larger or equal to 1 (one steady point)
- The nature of this point can be adjusted (from stable to unstable via centre)

# Behaviour at Infinity

A New Explosive Mode

• We could not prove simply that the trajectories remained bounded as time goes to infinity.
• Simulations quickly showed this was a hopeless effort
• For a combination of parameters trajectories are not bounded!!!

Case 1: When BC/D < 1 (the system has 3 steady points)

• trajectories are not bounded
• the predator population converges to a steady value D/C
• this predator population is too low to repress the prey population
• the prey population diverges to infinity

Case 2: When BC/D>=1 (the system has 2 steady points)

• trajectories remain bounded and spin around the second steady point
• we can apply Poincare-Bendixson
• consequently
- when the point is stable, the system stabilises itself at the steady point
- else we have oscillations around the steady point

Summary of the Results

It follows from the results stated above that the system has three different modes
• Prey Explosion
• Stability
• Oscillations

# Typical Simulations

First Mode: Prey Explosion

Phase Diagram
Time Diagram

Second Mode: Oscillations

Phase Diagram
Time Diagram

Last Mode: Stability

Phase Diagram
Time Diagram

• Note on the Simulations:
• Simulations using different initial conditions are assigned different colors (the open-end of the trajectories is the starting point).
• In the phase diagrams , red dots symbolise a steady points.

# Conclusion

• Bounding the predator growth has not removed the capacity of the system to oscillate on a unique limit-cycle.
• However, it has introduced a new dangerous (and highly unrealistic) mode where the prey population explodes.
• Fortunately a final modification still needs introducing: the use of a chemostat in our final construct. The chemostat is used to keep the ratio of cells constant primarily, but also to get rid of any excess in the system. It is hoped that this regularising behaviour will help stabilise the system enough to eliminate the 'explosion' mode.

# Appendices

A more detailed analysis of the system was made by Matthieu. Please consult it if you have any questions on the results on this page.