# IGEM:IMPERIAL/2006/project/Oscillator/project browser/Test Prey Construct/Modelling

### From OpenWetWare

< IGEM:IMPERIAL | 2006 | project | Oscillator | project browser | Test Prey Construct

Super Parts | Prey Construct | ||
---|---|---|---|

Actual Part | |||

Sub Parts | F2620 | C0261 | I13401 |

- Specifications
- Design
**Modelling**- Implementation
- Testing/Validation

## Model assumptions and relevance

- LuxR is at steady state
- A good approximation if LuxR gene is left in an active state while cells are growing in medium

- LuxR and AHL bind in a linear second order fashion
- This is not strictly true as LuxR and AHL bind using pseudo-Michaelis-Menten kinetics
- Gives a good approximation if LuxR is in excess of AHL (ie not saturated by AHL)
- Unlikely to occur all the time as LuxI levels increase to very high amounts

- LuxI can be ignored
- LuxI levels vary throughout, and it is these levels that are directly responsible for AHL production
- This could be a major flaw in the model

## Model description

## Model variables and parameters

Variables
| ||||

Name | Description | Initial Value | Confidence | Reference |
---|---|---|---|---|

AHL | Starting [AHL], must be >0 | 1 | Unknown | links |

Parameters
| ||||

Name | Description | Value | Confidence | Reference |
---|---|---|---|---|

AHL Degredation | Rate of AHL Degredation | Half-Life = 24 Hours | Known | links |

AHL Vmax | Maximum rate of production of AHL | 10 | Unknown | links |

AHL Kd | Value at which [Substrate] gives 50% Vmax | 1000 | Unknown | links |