IGEM:IMPERIAL/2006/project/Oscillator/project browser/Test Sensing Prey Construct
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< IGEM:IMPERIAL | 2006 | project | Oscillator | project browser
| Super Parts | Prey Construct | ||
|---|---|---|---|
| Actual Part |  T9002: Test Sensing Prey Construct | ||
| Sub Parts | F2620 | intermediate_part | intermediate_part |
- Specifications
- Design
- Modelling
- Implementation
- Testing/Validation
Motivations
- This testing part, the Test Sensing Prey Construct, is representing the sensitivity of the prey cell towards the prey molecule (AHL)
- This part allows us to quantise the activation of gene expression by different concentrations of AHL and is used to assay AHL
- This testing part allows us to investigate three parameters:
- How different AHL levels activate genes at a constant LuxR expression (The transfer function of pLuxR)
- How much AHL is present, by referring to the transfer function
- How LuxR levels effect gene activation
Part interface
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Expected Behavior and Performance
| Properties | Specifications | |
|---|---|---|
| Input Range(s) | Input range of the prey should cover the total dynamic range of the prey. | |
| Output Range | Output range of the PoPs can be any range, needs to be measurable with Wallac Victor III Fluorimeter. | |
| Transfer function(s) | 
| |
| Latency | to be characterized | |
| Robustness | ... | |
| Variability | Variability at the population level (day to day) should be as low as possible (10%) | |
Open issues
- list known issues
