IGEM:IMPERIAL/2007/Calendar/2007-7-26

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We are in Week 3 of the iGEM project.


July
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Contents

Proposals

Task List

Students

Supervisors

Today's Schedule

Name Alex Anthony Ben Cheuk Dirk James Jerry Lucas Maira Peixuan
0900
0930
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Evening


Questions

Debriefing

Tech Team

Most recent tasks to be undertaken:

  • Post Cell-free schedule on wiki
  • Start creating labbook interface - one interface partitioned into 4 elements: one for each project aspect: Cell-free, HRP, CBD & Biofilm
  • Reorganize projects page, sifting data into 3 sections namely:
  1. Discarded
  2. Approved
  3. Current

Access to labs/Experimentation

A concise record of possible hazardous procedures/materials employed, during the course of laboratory work, needs to be created and sent to Mr Dave Featherbe

In-Veso/Cell-Free System

  • Timetable of proposed tasks was created. See calendar section
  • Propose to have researcher give talk about vesicle formation?

Cell-by-Date

The following issues were considered:

  • Change of reporter system from that utilizing luciferase to that utilizing beta-galactosidase
    • Crucial: increase stability of reporter system and ensure that it performs an accumulative task
  • Look further into viable promoters to be used
  • In light of the above, it was proposed that short talks, concerning possible promoters & reporters, be incorporated into the Journal Club scheme

Biofilm

Issues raised:

  • Further reading/research is required to increase knowledge of this system
  • Reporter system employed: for test purposes initially GRP or RFP - to ensure functioning of system as whole. As confidence in system grows, a more effective reporter mechanism can be introduced e.g. luciferase

HRP

  • The required constructs were ordered
  • Determine, by the end of Friday, final 3 configurations of HRP system to pursue

Journal Club

It was determined, that the journal club be brought to life, on Monday, 30 July 2007. Two journals are to be presented: one on the cell-free system; another, on a reporter mechanism.

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