IGEM:IMPERIAL/2007/Calendar/2007-8-20
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We are in Week 7 of the iGEM project
Project Calendar
<calendar> name=IGEM:IMPERIAL/2007/Calendar date = 2007/08/01 view=threemonths format=%name/%year-%month-%day weekstart=7 </calendar>
Debrief
Modelling
- Got most equations for infector detector - up on the wiki
- Most simulations done
- Some problems, like with the constitutive promoter - will discuss with Captain ID and protocols team to resolve the issues
- Need to finish forming all the equations and highlight all the issues with the modelling of both ID and CBD by the end of the week
Vessicles
- Didn't form any vessicles today
- Got vessicles for sure, but GFP seems to be only around the membrane - possible reason: GFP was not put in the right quantity at the right step
- Will need to form vessicles again to verify
Protocols
- Reaction buffer needed for the home-made cell extract to work - ordered all the reagants
- Home-made cell extract tests put on hold - will carry on testing the DNA constructs in the commmercial one
- Tests will be done for pTet, PT7, GFP in vitro
Lab team
- IPTG solution made for the cell extract experiments
- Cloned a few more bricks, and re-cloned some that didn;t work earlier
Weekly digest
- Done and send to all the supervisors
