IGEM:IMPERIAL/2007/Calendar/2007-8-24

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We are in Week 7 of the iGEM project

Project Calendar

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Debrief

Modelling

  • Simulations almost completed - will be put on the wiki during the weekend
  • Compared the modelled graph of pLux construct with the one got by experiment - have the same general shape but no peek in the model
  • ID construct - problem with the constitutive promoter - need more data

Vesicles

  • Have small vesicles with GFP - have more in quantity than before but not entirely sure if they are actually vessicles
  • Need to decide whether to concentrate on gene expression or proof of concept - need more people in the vesicles team

Lab team

  • Finished makig the reaction mixture

Protocols

  • Tested homemade cell extract with the reaction buffer - doean't work
  • LuxR construct - works quite well in-vitro
  • Will test comercial cell extract with the homemade reaction buffer

Meeting with Prof. Kitney

  • Make presentations - make them see the big picture
  • Need to confirm length of meeting
  • Presentation teams:
    1. Veso - Dirk, Alex
    2. CBD - Alex, PPX, Ant, Lucas
    3. ID - Ben, Jerry, Maira, James
    4. Logo - Dirk, Ant
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