IGEM:IMPERIAL/2007/Calendar/2007-8-28

We are in Week 8 of the iGEM project

Project Calendar

<calendar> name=IGEM:IMPERIAL/2007/Calendar date = 2007/08/01 view=threemonths format=%name/%year-%month-%day weekstart=7 </calendar>

Debrief

Experiments

Phase 1

Inital testing of the biobricks

• pTet works
  • doesn't work below 10°C or above 45°C
  • fluorescence peak at 25°C - needed a monotonic relation between temperature and GFP expression
• pT7 - doesn't work - being re-cloned
• pLux - works at quite a high [AHL]
  • not very good fluorescence for a realistic [AHL]
  • no GFP for 1nM of AHL
  • possible to change the threshold of the construct?

Cell extract testing

• Home-made cell extract with home-made reaction buffer - doesn't work
  • Flooded the extract with DNA - still dosn't work
• Need more comercial cell extract - budget limit almost reached
  • Need to plan experiments more efficiently

Modelling

• Finished models for construct 1 and 2
• Currently documenting on the wiki
• Preparing for presentation

Vessicles

• Prepared presentation for Wednesday
• Done calculations for changes in protocols - to make the development of vessicles better
• Test again tomorrow
• Might have found a non-toxic method to create pores on vessicles - need further investigation
  • Will affect stability and life-span

Cloning of biobricks

• All working biobricks cloned
• Making substitutes for the ones not woorking
• New parts to be added to the registry
  • Waiting for dsRed to arrive
• No further ligations
• Purified LuxR - has been ordered (USA)
  • Will pursue both construct 1 and 2 for ID