IGEM:IMPERIAL/2007/Calendar/2007-8-30

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We are in Week 8 of the iGEM project

Project Calendar

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Debrief

Vesicles

Should the testing and characterisation of vesicles be pursued?

Reasons to abandon

  • Process of formation not very predictable - not even with same protocols
    • variation would affect characterisation
  • May not be able to carry out all tests for the applications
    • not enough time or resources (finances and equipment)
  • No method of counting vessicles - but can get less accurate results by population analysis
  • Found non-toxic pore protein - expensive
  • ID lagging in terms of testing of constructs - can use the man power in the lab
  • Would need a lot more man power to carry on research and do experiments - may still not finish on time!

Reasons to pursue

  • GFP may degrade after a few hours in-vitro - vessicles would increase life-span to ~ 3-4 days
    • life-span of at least a few days required for the CBD construct to fulfil current specification
    • but can modify the specification - threshold device rather than thermal history?
  • Haven't yet tried the cell extract in vessicles - may get better results!

Experiments

  • Phase 2 started - testing CBD constructs for temperature
  • Experiments going ok - but may run over in terms of the budget
  • ID construct - purification of LuxR may take time

Wiki

  • Tidy up 'public wiki' - seperate from 'working wiki'
  • Protocols hard to understand for outsiders - make more readable
  • Calender??
  • Anthony and Alex to re-design the wiki
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