IGEM:IMPERIAL/2007/Calendar/2007-9-11

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We are in Week 10 of the iGEM project


Project Calendar

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Debrief

Vesicles

  • Put pTet-GFP into vesicles - need to see what the result is tonight
  • Looking at equations relating expression in vesicles - to help with data analysis

Dry lab

  • Doing the wiki
    • graphic design

Experimental design (wet lab)

  • 24 hr experiment on CBD construct - put on hold
    • going to sort the methododlogy first
  • Design experiments to test affect of evaporation and how to reduce it
    • Mineral oil - possibility but affects oxygen supply too
  • Today testing:
    • if evaporation prevented by layer of oil
    • if layer of oil affects the reading of tehe fluorometer
  • Test will be done tomorrow at 37°C using either mineral or paraffin oil (depending on results from today's experiment)
    • Will test for affect on expression of the samples due to oil (blockage of oxygen)
  • T7 should be ready by tomorrow

Problems

  • Twinkle can't be moved from the teaching lab - need to talk to biochem lab to use theirs
  • DsRED express - will be delivered earlier next week (problem with quality control)
  • GFP mut-b3 from the lab - needs to be PCRed and cloned with a tag
  • LuxR
    • purity < 50%
    • Can't get exact [LuxR]
    • Need LuxR 10 x the amount of DNA for ID construct

CBD

Meeting scheduled with Vincent after tomorrow's debrief

  • Refocus CBD application/specification
    • Results so far don't comply with the specification
  • Alex - find out what is stopping expression of the pTet construct below 10 and over 45°C
  • Might be useful before meeting to get min working temperature
    • not possible as experiments put on hold until methodology improved
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