IGEM:IMPERIAL/2007/Calendar/2007-9-3

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We are in Week 9 of the iGEM project

Project Calendar

July
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August
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September
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Debrief

Testing of DNA constructs

  • Raw results up on the wiki
  • Refinfed data and graphs currently being put up
  • Data to be given to modelling team for analysis
  • Problems with staggering of experiments - results not very useful
    • Talk to supervisors
    • Try to get longer access to equipment (fluorometer, incubator etc)
    • Find out methods to reduce error and variability in results

Cloning

  • pTet and pLux constructs have been cloned
    • > 300µg of each DNA made - enough for all experiments
  • LuxR arrived - might be transformed by Wed
    • Protocol team to talk to clonning team about details
  • pT7-GFP construct being cloned in BL21

Vesicles

  • New procedure being adapted which works well- not leaving overnight and using 10ml suspension
    • Saves ingredients
    • No need to leave overnight
  • Cell extract will be tested in vesicles tomorrow
  • FACS machine for counting vesicles - not sure if can get access
  • No concrete decision yet made on where vesicles fit into the project
    • Meeting between Dirk (captain vesicles), Ben (captain ID) and Alex (captain CBD) to discuss feasibility of vesicles

Wiki

  • In the process of being redesigned - Alex and Anthony
    • Make wiki more organise and facilitate navigation for visitors
    • Talked to Vincent
    • Talking to individual groups about sections relevant to them and proposed layout

AOB

  • Characterisation of chassis - need to focus on it a bit more
    • Modelling and testing within in-vitro and vesicles
    • Use literature rather than experiments to characterise chassis being used
    • Use the information in in-veso teams(old oraganisation) page
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