IGEM:IMPERIAL/2007/Calendar/2007-9-6

We are in Week 9 of the iGEM project

Project Calendar

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Debrief

GFP problem

• Experimental design team had a meeting with Kirsten and Frank regarding the problem
• Have decided to concentrate on characterising DsRed Express
• All constructs will be cloned with DsRed Express and tested
  • Should be able to start experimenting on DsRed Express constructs by start of week 11
• In the mean while will carry on looking for purified form of GFP mut3B

Meeting with Paul Freemont

• 12pm tomorrow
• Each team to make an agenda to talk on
  • Must talk about what has been achieved in the past few weeks - no formal presentation

Problems with experments

• 24 hour access to lab and fluorometer to get better results as staggering not giving good results
• Freemont will arrange for a fluorometer that can be used 24 hours
  • No temperature control so can only carry out tests at room temperature and degradation experiments

Data analysis

• Dry lab team waiting for better results/data from the excperiments
• Current data not very useful - 24hr experiments would be very useful
• Currently writing a program to carry out data analysis

Cell extract

• Homemade cell extract was tested with pTet and pT7 constructs
  • Results seemed ok but fluorescence was seen in the negative controls too in a fluorescence microscope
  • Possible reasons - plates, the cell extract or the reaction mixture is contaminated
• Will carry out more tests on homemade cell extract to figure out where the fluorescence is from

Vesicles

• Characterising emulsion process - refining protocols to improve the process of vesicle formation
• Trying to come up with techniques to quantify the process of vesicle formation

French iGEM team members visit

• Hopefully the following people should be able to show them around and talk about the project:
  • Lucas
  • Alex
  • Anthony
  • Cheuk

Other issues

• Dry lab team should be free from modelling and data analysis by end of this week
  • Can join the vesicles team and/or the experimental design team
• A log book placed with the fluorometer
  • Need to write down full details on it about all the experiments being carried out - for record