IGEM:IMPERIAL/2007/Calendar/2007-9-6
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We are in Week 9 of the iGEM project
Project Calendar
<calendar> name=IGEM:IMPERIAL/2007/Calendar date = 2007/08/01 view=threemonths format=%name/%year-%month-%day weekstart=7 </calendar>
Debrief
GFP problem
- Experimental design team had a meeting with Kirsten and Frank regarding the problem
- Have decided to concentrate on characterising DsRed Express
- All constructs will be cloned with DsRed Express and tested
- Should be able to start experimenting on DsRed Express constructs by start of week 11
- In the mean while will carry on looking for purified form of GFP mut3B
Meeting with Paul Freemont
- 12pm tomorrow
- Each team to make an agenda to talk on
- Must talk about what has been achieved in the past few weeks - no formal presentation
Problems with experments
- 24 hour access to lab and fluorometer to get better results as staggering not giving good results
- Freemont will arrange for a fluorometer that can be used 24 hours
- No temperature control so can only carry out tests at room temperature and degradation experiments
Data analysis
- Dry lab team waiting for better results/data from the excperiments
- Current data not very useful - 24hr experiments would be very useful
- Currently writing a program to carry out data analysis
Cell extract
- Homemade cell extract was tested with pTet and pT7 constructs
- Results seemed ok but fluorescence was seen in the negative controls too in a fluorescence microscope
- Possible reasons - plates, the cell extract or the reaction mixture is contaminated
- Will carry out more tests on homemade cell extract to figure out where the fluorescence is from
Vesicles
- Characterising emulsion process - refining protocols to improve the process of vesicle formation
- Trying to come up with techniques to quantify the process of vesicle formation
French iGEM team members visit
- Hopefully the following people should be able to show them around and talk about the project:
- Lucas
- Alex
- Anthony
- Cheuk
Other issues
- Dry lab team should be free from modelling and data analysis by end of this week
- Can join the vesicles team and/or the experimental design team
- A log book placed with the fluorometer
- Need to write down full details on it about all the experiments being carried out - for record