IGEM:IMPERIAL/2007/DNA Constructs/Con Diagrams old
Here lies the ruins of the ancient constructs page
- Construct CBD 1
- Construct CBD 2
- Entire construct is currently in Biobricks Registry: BBa_I13522 (available), in pSB1A2 (AmpR)
- pTet can be used with E.coli RNAP in vitro and maybe in veso, and it gives constitutive expression.
- Construct CBD 3
- pBAD promoter with GFP is currently available in Biobricks Registry: BBa_J5528 (available), in pSB2K3 (KanR)
- Need Ara C regulator protein in vitro and in veso. Ara C available with the RBS:BBa_S03550
- pBad can be used with E.coli RNAP in vitro, and it is inducible by arabinose.
- To be used in veso, arabinose transport proteins have to be inserted in the phospholipid bilayer of the vesicles.
- Construct CBD 4
- Entire construct is currently available in Biobricks Registry: BBa_J07037 (avaiable), in pSB2K3 (KanR)
- pcI gives constitutive expression.
- Construct CBD 5
- Construct CBD 6
- T7 promoter is in the process of cloning
- dsRed has been ordered
- Construct CBD 7
- tet promoter available in the registry: BBa_R0040
- dsRed has been ordered
- Construct CBD 8
- Construct CBD 9
- λcI regulated promoter available in the registry:BBa_R0051
- Construct CBD 10
- Parts avaiable ....
Constructs for Infector Detector
- Construct ID 1
- Construct ID 2
- Entire part is currently in Biobricks Registry: BBa_J24677 (available), in pSB1A2 (AmpR)
- Entire part minus GFP reporter system is currently in Biobricks Registry: BBa_F2620 (available), in pSB1A2 (AmpR)
- GFP reporter system is currently in Biobricks Registry: BBa_I13504 (available)
- pLux can be used with E.coli RNAP in vitro and maybe in veso, and it is repressed by LuxR but activated by AHL:LuxR complex.
- Construct ID 3
- Construct ID 4
- Construct ID 5
This section contains irrelevant data and should be disregarded:
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|| || || BBa_I719??? || hrpR-hrpS & phrpL || Pending || hrpR-hrpS + phrpL || ID detector test construct 3 and final construct 5
|- style="background:#CCFFCC" align="center"
|| || || BBa_I719??? || Ptet-luxR-Plux & hrpR-hrpS-phrpL || Pending || [http://parts.mit.edu/registry/index.php/Part:BBa_F2620 BBa_F2620] + hrpR-hrpS-phrpL || ID detector test construct 3 and final construct 5
|- style="background:#CCFFCC" align="center"
|| || || BBa_I719??? || Ptet-luxR-Plux-hrpR-hrpS-phrpL & GFP || Pending ||Ptet-luxR-Plux-hrpR-hrpS-phrpL + [http://parts.mit.edu/registry/index.php/Part:BBa_E0240 BBa_E0240] || ID detector test construct 3
|- style="background:#CCFFCC" align="center"
|| || || BBa_I719??? || Ptet-luxR-Plux-hrpR-hrpS-phrpL & DsRed|| Pending ||Ptet-luxR-Plux-hrpR-hrpS-phrpL + DsRed || ID detector final construct 5