IGEM:IMPERIAL/2007/JournalClub

Hrp System

Alex and Maira

• "Enhancer-Binding Proteins HrpR and HrpS Interact To Regulate hrp-Encoded Type III Protein Secretion in Pseudomonas syringae Strains", Steven W. Hutcheson et al, Journal of Bacteriology, October 2001, p. 5589-5598, Vol. 183. link

Ben and Anthony

• "Whole-Genome Expression Profiling Defines the HrpL Regulon of Pseudomonas syringae pv. tomato DC3000, Allows de novo Reconstruction of the Hrp cis Clement, and Identifies Novel Coregulated Genes", Adriana O. Ferreira et al, MPMI Vol. 19, No. 11, 2006, pp. 1167–1179. DOI: 10.1094/MPMI-19-1167.

Cheuk and Dirk

• "Transcriptional Regulation of Components of the Type III Secretion System and Effectors in Pseudomonas syringae pv. phaseolicola", R. Thwaites et al, MPMI Vol. 17, No. 11, 2004, pp. 1250–125.

Peixuan and Jerry

• "Transcriptional regulation of the Pseudomonas aeruginosa type III secretion system", Timothy L. Yahr1* and Matthew C. Wolfgang, Molecular Microbiology (2006).

Day 1 (13 July 2007)

James and Jerry

1. Voigt CA. Genetic parts to program bacteria. Curr Opin Biotechnol. 2006 Oct;17(5):548-57. DOI:10.1016/j.copbio.2006.09.001 |

Day 2 (16 July 2007)

Cheuk and Maira

1. Andrianantoandro E, Basu S, Karig DK, and Weiss R. Synthetic biology: new engineering rules for an emerging discipline. Mol Syst Biol. 2006;2:2006.0028. DOI:10.1038/msb4100073 |

• PPT

Questions about this paper:

Q: How easy is it to modify part via directed evolution?
A: Difficulty is beyond the scope of the project.

Q: Any tips for multi-module design ?
A: Try to keep the number of modules small.

Day 3 (17 July 2007)

James and Lucas

• Idempotent Vector Design for Standard Assembly of Biobricks [Tom Knight,MIT Artificial Intelligence Laboratory, 2003]

Questions about this paper:

Q: How can we determine whether a plasmid has the insert?
A: If the insert disrupts a gene in the plasmid that codes for an antibiotic resistance, the bacteria with the correct recombinant plasmid will be susceptible to the antibiotic on the replica plate.