IGEM:IMPERIAL/2007/Notebook/2007-10-2

From OpenWetWare

Jump to: navigation, search

Evaporation experient

  • We carried on experiments from yesterday to find a method to reduce evaporation of samples
  • Results???

Test purified luxR

  • Aim: The aim of this experiment was to test if the purified luxR works and if the construct 2 works
  • To test the purified luxR we used these protocols
  • The results of this experiment can be found here
  • The purified LuxR appeared to be functional in vitro, however there were stability issues that reduce the synthesis of GFPmut3b when pLux-GFPmut3b is used with purified LuxR.
  • For the characterisation of the infector detector system the pTet-LuxR-pLux-GFPmut3b (construct 1) will be used.

Test pT7 in-vivo

  • Aim: The aim of the test was to verify that pT7-GFP expresses in-vivo
  • The test was carried out using these protocols??
  • The results can be found here??
Personal tools