IGEM:IMPERIAL/2007/Notebook/2007-8-1

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August
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Preparation of Stock Solutions

  • 1x 1 litre of 10x TBE
  • 20x 100 ml of LB media in conical flasks

Preparation of Competent Cells

  1. Poured LB agar plates
    • Nalixidic acid (15 μg/ml)
    • Tetracyclin (20 μg/ml)
  2. Streaked E.coli cells on agar plates
    • DH5Alpha
    • XL1Blue
  3. Incubated plates at 37°C overnight

Preparation of Equipment

  • 1x 37°C shaking incubator
  • 1x 4°C fridge
  • 1x -20°C freezer
  • 2x Weighing scales
  • 3x Magnetic stirrer
  • 3x Microcentrifuge
  • 3x Vortex machines
  • 2x UV Spectrometer
  • 5x Gilson pipettes (P1000, P200, P20, P10, P2)
  • 20 boxes x Tips (yellow and blue)
  • 5 boxes x Small tips
  • Eppendorf tubes
  • Centrifuge tubes
  • 50x 5 ml pipettes
  • 50x 10 ml pipettes
  • 50x 25 ml pipettes
  • 5x Pipette fillers
  • 37°C water bath
  • 15°C water bath
  • Driblock
  • 10 packets x Innoculation loops
  • 3x Bunsen burners
  • 3 boxes Matches
  • 3x Agarose gel equipments
  • Microwave oven
  • Transilluminator
  • Camera
  • 3x Scapel (for cutting agarose gel)
  • 1 litre bottles
  • Beakers
  • Conical flasks
  • Volumetric flasks
  • Measuring cylinders
  • Gloves
  • Goggles
  • Markers
  • Sticker labels
  • Paper towels
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