IGEM:IMPERIAL/2007/Notebook/2007-8-2

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August
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567891011
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September
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30
October
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28293031


Preparation of Stock Solutions

  • 1x 1 litre of 1M Tris-Cl
  • 1x 500 ml of 50x Tris-acetate

Preparation of Competent Cells

  1. Picked colonies from agar plates
  2. Grew colonies in LB media
    • DH5Alpha
    • XL1Blue
  3. Incubated cultures at 37°C overnight
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