IGEM:IMPERIAL/2007/Notebook/2007-8-2
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<calendar> name=iGEM:IMPERIAL/2007/Notebook date=2007/09/2 view=threemonths format=%name/%year-%month-%day weekstart=7 </calendar>
Preparation of Stock Solutions
- 1x 1 litre of 1M Tris-Cl
- 1x 500 ml of 50x Tris-acetate
Preparation of Competent Cells
- Picked colonies from agar plates
- Grew colonies in LB media
- DH5Alpha
- XL1Blue
- Incubated cultures at 37°C overnight