IGEM:IMPERIAL/2007/Notebook/2007-8-3

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Preparation of Stock Solutions

  • 2x 1 litre of M9 media
  • 2x 1 litre of LB media
  • 4x 500 ml of LB media in conical flasks

Preparation of Antibiotics Plates

  • 30x LB + Amp plates
  • 30x LB + Kan plates
  • 30x LB + Amp + Kan plates
    • 50mg/ml Amplicillin solution
    • 10mg/ml Kanamycin solution

Preparation of Competent Cells

  1. DH5alpha and XL1Blue were made electrocompetent

Protocols can be found at Preparation of Competent Cells in the general protocols page

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