IGEM:IMPERIAL/2007/Notebook/2007-8-31

From OpenWetWare

Jump to: navigation, search
August
SMTWTFS
1234
567891011
12131415161718
19202122232425
262728293031
September
SMTWTFS
1
2345678
9101112131415
16171819202122
23242526272829
30
October
SMTWTFS
123456
78910111213
14151617181920
21222324252627
28293031


Contents

Cell by date - Operating Temperature Range (continue)

Construct - pTet-GFP BBa_I13522
Temperatures - 37 °C and 20°C Aims: To test for the behaviour of the DNA construct (pTet) at temperatures 20oC and 37oC by observing the amount of fluorescence produced over a period of 24 hours.

  • Measurements was carried out from the samples incubated overnight.

Protocol can be found here under Phase 2-Operating Temperature Range on the experimental design page.

Results can here under Results on the experimental design page.


Preliminary AHL testing

Aim: This experiment was done to test the response time and steady state of the pTet-LuxR-pLux-GFP DNA construct at the different AHL concentrations, ranging from 100nM, 50nM, and 10nM.

Protocol can be found here under Phase 2-Preliminary AHL testing on the experimental design page.

Results can here under Results on the experimental design page.


GFP Calibration Curve

Aim: This experiment was done to generate a calibration curve of the amount of fluorescence generateed with respect to the concentration of GFP in the solution.

Protocol can be found here under Phase 2-GFP calibration curve on the experimental design page.

Results can here under Results on the experimental design page.

Construction of pT7-GFP

  1. Transformed ligated plasmid into electrocompetent DH5alpha cells

Protocols can be found at Transformation in the general protocols page

  1. Grew cells at 37°C for 30 min
  2. Plated onto Kanamycin plates
  3. Left plates overnight at 37°C
Personal tools