IGEM:IMPERIAL/2007/Notebook/2007-9-10

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Vesicles

Results

Sample 8, produced on 04.09.2007 was looked at again under the fluorescence microscope, but this time only a single 2μm vesicle was found.

Preparations

Three POPC/dodecane 10ml suspensions were prepared.

Cloning of Biobricks (for Maxiprep)

  1. Placed 100 ml of LB in a 500 ml conical flask
  2. Innoculated 1 ml of culture into LB media in flask
  3. Incubated overnight at 37 °C

  • 25. BBa_J37032 [pLux-GFP]
  • 13. BBa_B0015 [ ]

Protocols can be found at Maxiprep in the general protocols page

Transformation of Biobricks

  1. 1 biobrick was transformed into XL1-Blue
  2. Cells were grown for half an hour and plated
  3. Plates were incubated at 37°C overnight
  • Transformed in XL1-Blue
    • pSB1A3 [pSB1A3] (re-clone)

Protocols can be found at Electroporation in the general protocols page

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